| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 671 | Clostridium tetani | GAAGGCAATCTGAACTGCACT | ATGCCGAACATTCCGCATC | 58.84 | 58.98 | 293 | 71 |
100.00%
|
100.00%
|
| Primer 672 | Clostridium tetani | TCGGGAACTGATGGAGTGG | AAGCCAGGCATTTCCGCA | 58.71 | 60.28 | 260 | 71 |
100.00%
|
100.00%
|
| Primer 673 | Clostridium tetani | GAACTGATGGAGTGGGCACT | AAGCCAGGCATTTCCGCA | 59.67 | 60.28 | 256 | 71 |
100.00%
|
100.00%
|
| Primer 674 | Clostridium tetani | AACTGATGGAGTGGGCACT | AAGCCAGGCATTTCCGCA | 58.53 | 60.28 | 255 | 71 |
100.00%
|
100.00%
|
| Primer 675 | Clostridium tetani | TTGCTGCCCATCTTCCAGG | GAGCTGCTAACATACCTGCAT | 60.00 | 58.16 | 180 | 71 |
100.00%
|
100.00%
|
| Primer 676 | Clostridium tetani | TCCAAGACTAGGTTGTGGCA | TCTTGTTCAGCCCAGTTTGC | 58.57 | 58.97 | 240 | 71 |
100.00%
|
100.00%
|
| Primer 677 | Clostridium tetani | GGACAGAGCATAGGTGTGGA | ACGGCATCAGGGTCAACT | 58.80 | 58.52 | 212 | 71 |
100.00%
|
100.00%
|
| Primer 678 | Clostridium tetani | AGGACAGAGCATAGGTGTGG | ACGGCATCAGGGTCAACT | 58.80 | 58.52 | 213 | 71 |
100.00%
|
100.00%
|
| Primer 679 | Clostridium tetani | AGGACAGAGCATAGGTGTGGA | ACGGCATCAGGGTCAACT | 60.27 | 58.52 | 213 | 71 |
100.00%
|
100.00%
|
| Primer 680 | Clostridium tetani | ACAGGAGATGGCGATGGTG | TCCTTCTCCAACAGCTGCA | 59.48 | 58.86 | 285 | 71 |
100.00%
|
100.00%
|